Development of real-time PCR for detection of <Emphasis Type="Italic">Mycoplasma hominis</Emphasis> |
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Authors: | Email author" target="_blank">Agata?BaczynskaEmail author Helle?F?Svenstrup Jens?Fedder Svend?Birkelund Gunna?Christiansen |
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Institution: | 1.Department of Medical Microbiology and Immunology,Aarhus University,Aarhus C,Denmark;2.The Fertility Clinic,Br?dstrup Hospital,Br?dstrup,Denmark |
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Abstract: | Background
Mycoplasma hominis is associated with pelvic inflammatory disease, bacterial vaginosis, post partum fever, sepsis and infections of the central
nervous system often leading to serious conditions. Association with development of female infertility has also been suggested,
but different publications present different results. We developed a sensitive and fast diagnostic real-time PCR to test clinical
samples from women undergoing laparoscopic examination before fertility treatment. To develop a test for the detection and
quantification of M. hominis we selected a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (gap), as a target. |
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Keywords: | |
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