紫外照射神经酰胺的生成及神经酰胺引起的细胞凋亡

参照文献报道的方法建立了测定细胞神经酰胺的激酶催化法，以该法所做的标准曲线在一定浓度范围内有很好的线性关系。以ＵＶＢ照射ＮＩＨ ３Ｔ３细胞，可以使细胞神经酰胺的水平在１分钟之内迅速升高１．５倍以上，基本上达到了细胞所能生成神经酰胺的最高峰值。可细胞膜的外源性神经酰胺（Ｃ２－ｃｅｒａｍｉｄｅ）作用于ＮＩＨ ３Ｔ３细胞后能够导致细胞的凋亡和坏死，其凋亡率在一定剂量范围内随神经酰胺浓度的增加而增加；而在

CERAMID GENERATED FROM ULTRAVIOLET IRRADIATION AND IT INDUCED APOPTOSIS

The method to detect ceramide within cells was established in our laboratory and the standard curve produced by the method appeared in a good linear correlation with ceramide in a certain range. The concentration of ceramide produced by NIH 3T3 cells after UVB irradiation was 2.5 times as that of the control within 1 minute. That is nearly to the highest concentration which can be produced after UVB irradiation. Exogenous ceramide (C2-ceramide), a cell-permeable analogue of ceramide, could induce NIH 3T3 cells apoptosis as well as necrosis which is dose- and time-dependent.