Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density |
| |
Authors: | Paulo R H Moreno Juriaan E Schlatmann Robert van der Heijden Walter M van Gulik Hens J G ten Hoopen Robert Verpoorte Joseph J Heijnen |
| |
Institution: | (1) Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA Leiden, The Netherlands;(2) Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands |
| |
Abstract: | In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in moles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high-and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities.Biotechnology Delft Leiden, Project Group Plant Cell Biotechnology
Correspondence to: R. Verpoorte |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|