Evidence of Gene Conversion Events Between Paralogous Sequences Produced by Tetraploidization in Salmoninae Fish |
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Authors: | Bernard Angers Karim Gharbi Arnaud Estoup |
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Institution: | (1) Laboratoire de Génétique des Poissons, INRA, Jouy-en-Josas, France, FR |
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Abstract: | We investigated the occurrence of gene conversions between paralogous sequences of Salmoninae derived from ancestral tetraploidization
and their effect on the evolutionary history of DNA sequences. A microsatellite with long flanking regions (750 bp) including
both coding and noncoding sequences was analyzed. Microsatellite size polymorphism was used to detect the alleles of both
paralogous counterparts and infer linkage arrangement between loci. DNA sequencing of seven Salmoninae species revealed that
paralogous sequences were highly differentiated within species, especially for noncoding regions. Ten gene conversion events
between paralogous sequences were inferred. While these events appears to have homogenized regions of otherwise highly differential
paralogous sequences, they amplified the differentiation among orthologous sequences. Their effects were larger on coding
than on noncoding regions. As a consequence, noncoding sequences grouped by orthologous lineages in phylogenetic trees, whereas
coding regions grouped by taxa. Based upon these results, we present a model showing how gene conversion events may also result
in the PCR amplification of nonorthologous sequences in different taxa, with obvious complications for phylogenetic inferences,
comparative mapping, and population genetic studies.
Received: 11 October 2000 / Accepted: 18 September 2001 |
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Keywords: | : Gene conversion — Microsatellite — Phylogeny — Recombination — Tetraploidization |
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