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Hemagglutinating activity of the fungusLentinus edodes (Berk.) sing [Lentinus edodes (Berk.) Pegler]
Authors:O M Tsivileva  V E Nikitina  L V Garibova  L A Zav’yalova  V V Ignatov
Institution:(1) Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, 410015 Saratov, Russia;(2) Present address: Department of Mycology and Algology, Biological Faculty, Moscow State University, 119899 Vorob’evy gory, Moscow, Russia
Abstract:The hemagglutinating (HA) activity of the submerged mycelium and the culture liquid (CL) of four strains ofLentinus edodes was studied. The HA activity of the CLs proved to be much higher than that of mycelia. The carbohydrate specificity of fungal agglutinating factors was determined. HA activity was investigated as a function of the inoculum size, cultivation temperature, and culture age. The agglutinating activity of different morphogenetic structures ofL. edodes F-249, including mycelium, brown mycelial mat (MM), primordia, and fruiting bodies, was studied. MM was found to possess the maximum HA activity, which can be explained by the possible involvement of agglutinins in the formation of MM, which is composed of glued hyphae.
Keywords:hemagglutinating activity  agglutinins of higher fungi  submerged mycelium            Lentinus edodes            cultivation conditions  fruiting bodies
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