Neuropeptide F and its expression in the yellow fever mosquito,Aedes aegypti |
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Authors: | Stanek Dawn M Pohl Jan Crim Joe W Brown Mark R |
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Affiliation: | Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA. |
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Abstract: | A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal. |
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