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Isolation of a Repeated DNA Probe Showing Polymorphism among Meloidogyne incognita Populations
Authors:P. Castagnone-Sereno  C. Piotte  P. Abad  M. Bongiovanni  A. Dalmasso
Abstract:Several Meloidogyne incognita geographic populations were characterized by analysis of the restriction fragment length polymorphisms (RFLP) obtained after digestion of their total DNA and hybridization with a [³²P]-labeled probe. The probe consisted of a 1.7-kb-repeated DNA sequence, isolated from a M. incognita genomic library, that hybridized to multiple BamH I fragments in the genome of each isolate. The patterns showed sufficient polymorphism to enable the accurate differentiation of all the populations tested.
Keywords:DNA   hybridization   intraspecific differentiation   Meloidogyne incognita   repetitive DNA
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