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槲蕨配子体形态发育的研究
引用本文:徐艳,李杨,李东,刘燕,檀龙云,刘保东,石雷.槲蕨配子体形态发育的研究[J].热带亚热带植物学报,2009,17(1):12-16.
作者姓名:徐艳  李杨  李东  刘燕  檀龙云  刘保东  石雷
作者单位:1. 中国科学院植物研究所,北京,100093;北京林业大学园林学院,北京,100083
2. 中国科学院植物研究所,北京,100093;中国科学院研究生院,北京,100049
3. 中国科学院植物研究所,北京,100093
4. 北京林业大学园林学院,北京,100083
5. 哈尔滨师范大学生命科学与技术学院,哈尔滨,150025
基金项目:国家科技部资助项目,中国科学院重要方向性项目,北京市自然科学基金 
摘    要:用无机培养基和土壤培养基分别培养槲蕨(Drynaria roosii Nakaike)孢子,显微镜下观察孢子萌发及配子体形态发育过程.结果表明:孢子黄色,具单裂缝,赤道面观豆形,极面观椭圆形,不具周壁,孢子外壁局部具大小不一的颗粒状纹饰.接种后10~12 d孢子萌发,萌发类型为书带蕨型,原叶体发育为槲蕨型.接种后20 d左右发育为片状体,片状体形成顶端细胞的时间较晚.毛状体出现在片状体形成之后,数量丰富,多为单细胞,分布于原叶体背腹面及边缘.接种后60 d左右发育形成幼原叶体,成熟原叶体呈心脏形.接种后65 d左右开始有性器官出现,精子器的出现较颈卵器早10 d左右.颈卵器成熟后,颈部常向原叶体基部倾斜或弯曲.原叶体受精后幼胚突破颈卵器生长.

关 键 词:槲蕨  孢子萌发  配子体发育
收稿时间:2008/1/11 0:00:00
修稿时间:9/9/2008 12:00:00 AM

Observation on the Gametophyte Development of Drynaria roosii Nakaike
Xu Yan,Li Yang,Li Dong,Liu Yan,Tan Longyun,Liu Baodong and Shilei.Observation on the Gametophyte Development of Drynaria roosii Nakaike[J].Journal of Tropical and Subtropical Botany,2009,17(1):12-16.
Authors:Xu Yan  Li Yang  Li Dong  Liu Yan  Tan Longyun  Liu Baodong and Shilei
Institution:1. Institute of Botany;the Chinese Academy of Science;Beijing 100093;China;2. College of Landscape Architecture;Beijing Forestry University;Beijing 100083;3. Graduate University of Chinese Academy of Sciences;Beijing 100049;4. College of Life Science and Technology;Harbin Normal University;Harbin 150025;China
Abstract:The spores of Drynaria roosii Nakaike were cultured in inorganic medium and soil, respectively. The spore germination and gametophyte development were observed under microscope. The results showed that the spores were yellowish, monolete, without perispore and bearing granular ornamentation on the exospore. They are bean-shaped in equatorial view and elliptical in polar view. The spores germinated within 10~12 days after inoculation, the type of germination is Vittaria-type while that of gametophyte develop...
Keywords:Drynaria roosii Nakaike  Spore germination  Gametophyte development  
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