Effect of storage of domestic cat (Felis catus) epididymides at 5 °C on sperm quality and cryopreservation |
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| Institution: | 1. Grupo de Ecología y Biología de la Reproducción, Museo Nacional de Ciencias Naturales (CSIC), Madrid, Spain;2. Royal Veterinary College, London, United Kingdom;1. Clinic for Reproduction and Large Animals, Veterinary Faculty, University of Ljubljana, Gerbi?eva 60, 1000 Ljubljana, Slovenia;2. Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Gerbi?eva 60, 1000 Ljubljana, Slovenia;3. Institute for Preclinical Sciences, Veterinary Faculty, University of Ljubljana, Gerbi?eva 60, Ljubljana, Slovenia;1. CECA/ICETA – Animal Sciences Centre, ICBAS – Abel Salazar Biomedical Institute, University of Porto, Portugal;2. Assisted Reproduction Unit, Minimally Invasive Surgery Centre Jesús Usón (CCMIJU), Cáceres, Spain;1. Departamento de Medicina Animal, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil;2. Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden;3. Departamento de Clínicas Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), Pelotas, Rio Grande do Sul, Brazil;4. Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil;5. Curso de Medicina Veterinária, Universidade Luterana (ULBRA), Canoas, Rio Grande do Sul, Brazil |
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| Abstract: | Postmortem sperm recovery from the epididymides may constitute a powerful tool for the conservation of valuable genetic material. The domestic cat (Felis catus) is a good model for wild felids and, using this model, we have explored the effect of epididymides storage time on sperm motility and percentage of intact acrosomes upon sperm recovery and after cryopreservation. We also examined the effect of time of sperm equilibration with glycerol before freezing on sperm motility and the percentage of intact acrosomes. Motility varied between sperm recovered from epididymides that were stored for different times. Significant differences were seen in the sperm motility index (SMI) before freezing (55.91 ± 2.02, 48.21 ± 1.47, and 43.03 ± 1.32) and after thawing (51.81 ± 3.02, 41.90 ± 2.14, and 42.35 ± 1.95) of sperm recovered from epididymides stored for 0, 48, or 72 h, respectively. The percentage of intact acrosomes did not vary significantly with storage time (average 60.33 ± 1.38% before and 52.50 ± 1.91% after freezing, respectively). The percentage of normal sperm after different storage times did not differ (average 19.22 ± 1.25% normal sperm after recovery). When epididymides were stored for 72 h, time of sperm equilibration with glycerol (30 vs. 120 min) resulted in significant differences in both motility (SMI = 39.17 ± 2.76 and 45.00 ± 2.65, respectively) and the percentage of intact acrosomes (45.76 ± 4.91% and 60.67 ± 3.64%, respectively) after thawing. In conclusion, best results are achieved when sperm are recovered from epididymides within 24 h of cool storage and when they are equilibrated with glycerol during 120 min before freezing. The current results should be useful in the further development of techniques for the rescue and cryostorage of epididymal spermatozoa of endangered felids. |
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