A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels |
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| Institution: | 1. Flinders Centre for NanoScale Science and Technology, Flinders University, PO Box 2100, Adelaide, SA 5001, Australia;2. Commonwealth Scientific and Industrial Research Organization (CSIRO), Materials Science and Engineering, Clayton, VIC 3169, Australia;3. Australian Nuclear Science and Technology Organization, New Illawarra Rd, Lucas Heights, NSW 2234, Australia;4. School of Chemistry and Bio21 Institute, The University of Melbourne, Parkville, VIC 3010, Australia;1. Department of Electrical and Electronics Engineering, Adhiparasakthi College of Engineering, Kalavai-632506, India;2. Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore-632115, India;3. Centre for Ocean Research, Sathyabama University, Chennai-600119, India;4. Department of Physics, Sathyabama University, Chennai-600119, India;1. Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo, Tokyo 112-8551, Japan;2. Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467, USA;3. JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan;1. Department of Oncology, University of Cambridge, Cambridge CB2 0QQ, UK;2. Clinical Trials and Statistics Unit, The Institute of Cancer Research, London, UK;3. Department of Radiation Oncology, Institut Curie, Paris, France;4. Université Paris Sciences et Lettres, Paris, France;1. University Technology Malaysia, Laser Center, Ibnu Sina Institute for Scientific and Industrial Research, Johor Bahru, Johor, 81310, Malaysia;2. Universiti Teknologi Malaysia, Department of Physics, Faculty of Science, Johor Bahru, Johor, 81310, Malaysia;3. Al-Qadisiyah University, Faculty of Education, Physics Department, Diwaniya 00964, Iraq;4. Al-Mustansiriya University, Faculty of Science, Physics Department, Baghdad 00964, Iraq |
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| Abstract: | A rapid, sensitive fluorescence measurement method for detecting the bacterial count using CdSe/ZnS as a fluorescence marker was described. High-quality CdSe/ZnS nanocrystals were synthesized and successfully conjugated with bacteria. The fluorescence intensity was proportional to bacterial count in the range of 102–108 CFU/mL and the low detection limit was 102 CFU/mL. |
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