Freeze-thawing as the factor of spontaneous activation of spermatozoa motility in common carp (Cyprinus carpio L.) |
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| Affiliation: | 1. Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland;2. Department of Ichthyology, Faculty of Environmental Sciences, University of Warmia and Mazury, ul. Oczapowskiego 2, 10-719 Olsztyn, Poland;3. Department of Aquaculture, The Stanislaw Sakowicz Inland Fisheries Institute, Olsztyn, Poland;4. Team Domestication in Aquaculture, UR AFPA, University of Lorraine, F-54000 Nancy, France;5. Department of Aquaculture, Szent István University, Gödöllő, Hungary;1. Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland;2. Institute of Ichthyobiology and Aquaculture of Polish Academy of Science Gołysz, 43-520 Chybie, Poland;1. Department of Fisheries, Graduate School of Agriculture, Kindai University, Naka-machi, Nara 631-8505, Japan;2. National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Minami-Ise, Mie 516-0193, Japan |
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| Abstract: | In the present study, we investigated the possibility of spontaneous carp spermatozoa activation by freeze-thawing. To evaluate this, the parameters of spermatozoa motility percentage, velocity, ATP content level and fertility rate of sperm were used. The motility and velocity of spermatozoa activated by freeze-thawing were characterized by motile spermatozoa with a median value of 16% and a velocity of 98 μm/s. In addition, the motility and velocity of sperm from the thawed samples were significantly lower than in the control (median value of 100% for sperm motility and 175 μm/s for sperm velocity). Furthermore, a spontaneously activated spermatozoa motility terminated within five minutes post-thaw time. After freeze-thawing the ATP level significantly decreased with post-thaw time (46 nmol ATP/109 and 10 nmol ATP/109 at 25 s and 10 min after thawing, respectively). Fertility of spermatozoa was not significantly affected within 10 min post-thaw. On the other hand, the fertility of frozen-thawed sperm was significantly lower if compared to fresh sperm. We conclude that the freeze-thawing procedure spontaneously activated spermatozoa motility in common carp. However, this activation did not negatively affect the fertility of frozen-thawed sperm. |
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