A rapid and efficient method for regeneration of plantlets from embryo explants of cumin (Cuminum cyminum) |
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| Authors: | Ebrahimie Esmaeil Habashi A.A. Ghareyazie B. Ghannadha M. Mohammadie M. |
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| Affiliation: | (1) Department of Tissue Culture and Transformation Agricultural Biotechnology Research Institute of Iran, (ABRII), P.O. Box 31535-1897, Karaj, Iran;(2) Department of Crop Production and Plant Breeding, University of Tehran, Karaj, Iran |
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| Abstract: | A new, simple and efficient method was developed for multiple shoot regeneration of cumin from imbibed embryo cultures. This method yielded a large number of shoots within short period of time (30–50 days) without any subculturing. The effects of different media, different embryo explants and various combinations of plant growth regulators (PGRs) on callus formation and shoot regeneration in cumin were investigated. Simultaneous callus formation and shoot regeneration was obtained. The best response for multiple shoot regeneration was observed on B5 medium containing 1.0 mg l–1 BAP, 0.2 mg l–1 NAA and 0.4 mg l–1 IAA, with an average of 140 shoots per explant. |
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| Keywords: | cumin embryo explant medicinal plants multiple shoot regeneration simultaneous callus formation and shoot regeneration |
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