A simplified hydroethidine method for fast and accurate detection of superoxide production in isolated mitochondria |
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| Authors: | Back Patricia Matthijssens Filip Vanfleteren Jacques R Braeckman Bart P |
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| Affiliation: | Laboratory for Aging Physiology and Molecular Evolution, Department of Biology, Ghent University, 9000 Ghent, Belgium. |
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| Abstract: | Because superoxide is involved in various physiological processes, many efforts have been made to improve its accurate quantification. We optimized and validated a superoxide-specific and -sensitive detection method. The protocol is based on fluorescence detection of the superoxide-specific hydroethidine (HE) oxidation product, 2-hydroxyethidium. We established a method for the quantification of superoxide production in isolated mitochondria without the need for acetone extraction and purification chromatography as described in previous studies. |
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