Lactate conversion to acetate,CO2 and H2 in cell suspensions of Desulfovibrio vulgaris (Marburg): indications for the involvement of an energy driven reaction |
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| Authors: | Ishverlal P Pankhania Alfred M Spormann W Allan Hamilton Rudolf K Thauer |
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| Institution: | (1) Mikrobiologie, Fachbereich Biologie, Phillipps-Universität, Karl-von-Frisch-Strasse, D-3550 Marburg, Federal Republic of Germany;(2) Present address: Department of Genetics and Microbiology, University of Aberdeen, Marischal College, AB9 1AS Aberdeen, Scotland, UK |
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| Abstract: | Cell suspensions of Desulfovibrio vulgaris were found to catalyze, in the absence of sulfate, the complete conversion of 1 lactate to 1 acetate, 1 CO2, and 2 H2 ( G 0=-8.8 kJ/mol) and of 1 pyruvate to 1 acetate, 1 CO2, and 1 H2 (G0=-52 kJ/mol). Protonophores, the proton translocating ATPase inhibitor N,N-dicyclohexylcarbodiimide, and arsenate specifically inhibited H2 formation from lactate but not from pyruvate. The results suggest that lactate oxidation to pyruvate and H2 (G
0=+43.2 kJ/mol) is energy driven. |
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| Keywords: | Lactate oxidation Pyruvate oxidation H2 formation Protonophores Dicyclohexylcarbodi-imide Arsenate Sulfate-reducing bacteria Desulfovibrio vulgaris |
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