Analysis of the occurrence and distribution of tobacco rattle virus in field soil and disease in a subsequent tulip crop |
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Authors: | ANTOON T PLOEG FRANS C ZOON JOOP DE BREE CEES J ASJES |
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Institution: | Research Institute for Plant Protection (IPO-DLO), PO Box 9060, 6700 GW Wageningen, The Netherlands;Agricultural Mathematics Group (GLW-DLO), PO Box 100, 6700 AC Wageningen, The Netherlands;Bulb Research Centre, PO Box 85, 2160 AB Lisse, The Netherlands |
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Abstract: | The occurrence and distribution of tobacco rattle virus (TRV) in field plots was determined by soil bait-testing and disease incidence in tulips subsequently grown on these plots was studied. The virus occurred in patches, calculated as 1.5 m × 3.6 m. The presence of virus was not correlated with numbers of potential vector trichodorid nematodes. Of three trichodorid nematode species present, only Paratrichodorus teres transmitted TRV which, as with virus isolates obtained in bait-tests and from infected tulips, reacted in serological tests with an antiserum prepared against a Dutch isolate of pea-early browning virus (PEBV). Virus prevalence in a subsequent tulip crop was 0.8% and in a sample of tulip plants, virus was recovered only from plants showing virus symptoms. Plots from which TRV was recovered in bait-tests yielded significantly more virus diseased tulips than plots which tested negative for virus. Growing bait-plants in field-plots, as compared with greenhouse tests using soil collected as a series of sub-samples, resulted in an underestimate of the occurrence of TRV. |
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Keywords: | Soil bait-tests tobacco rattle virus detection trichodorid nematodes Paratrichodorus teres transmi |
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