Identification of barley and wheat cDNA clones related to the high-Mr polypeptides of wheat gluten |
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Authors: | J Forde BG Forde RP Fry M Kreis PR Shewry BJ Miflin |
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Institution: | Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, England |
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Abstract: | We have identified 3 cDNA clones related to the high-Mr group of storage proteins in barley endosperm, the D-hordeins. A cDNA library has been constructed from wheat endosperm poly(A+)-RNA and screened using one of the D-hordein cDNA clones. Two wheat clones which cross-hybridised to the barley clone have been identified, by hybrid-release translation and nucleotide sequence analysis, as partial copies of mRNAs encoding the high-Mr gluten polypeptides of wheat. |
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Keywords: | D-Hordein Molecular cloning DNA-sequencing |
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