Improving tobacco freezing tolerance by co-transfer of stress-inducible CbCBF and CbICE53 genes |
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Authors: | P. Lin C. Shen H. Chen X. H. Yao J. Lin |
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Affiliation: | 1.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang, Zhejiang,P.R. China;2.Institute of Plant Biology, School of Life Sciences,Fudan University,Shanghai,P.R. China |
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Abstract: | Cold stress is one of the major limitations to crop productivity worldwide. We investigated the effects of multiple gene expression from cold tolerant Capsella bursa-pastoris in transgenic tobacco (Nicotiana tabaccum) plants. We combined CblCE53 and CbCBF into a reconstruct vector by isocaudomers. Plant overexpression of CbICE53 under the stress inducible CbCOR15b promoter and CbCBF under a constitutive promoter showed increased tolerance to both chilling and freezing temperatures in comparison to wild-type plants, according to the electrolyte leakage and relative water content. The expressions of endogenous cold-responsive genes in transgenic tobacco (NtDREB1, NtDREB3, NtERD10a and NtERD10b) were obviously upregulated under normal and low temperature conditions. These results suggest that the CbICE53 + CbCBF transgenic plants showed a much greater cold tolerance as well as no dwarfism and delayed flowering. Thus they can be considered as a potential candidate for transgenic engineering for cold tolerant tobacco. |
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