Retracted: Upregulated microRNA-15b alleviates ovarian cancer through inhitbition of the PI3K/Akt pathway by targeting LPAR3 |
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Authors: | Guang-Cai Li Xu-Ling Qin Huai-Hua Song Ying-Ni Li Yu-Yan Qiu Shi-Chang Cui Yong-Sheng Wang Hui Wang Jun-Ling Gong |
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Affiliation: | 1. Department of Obstetrics and Gynecology, Linyi People's Hospital, Linyi, People's Republic of China;2. Department of Emergency, Economic and Technological Development Zone People's Hospital of Linyi, Linyi, People's Republic of China;3. Department of Obstetrics and Gynecology, The Third People's Hospital of Linyi, Linyi, People's Republic of China;4. Department of Obstetrics and Gynecology, Economic and Technological Development Zone People's Hospital of Linyi, Linyi, People's Republic of China;5. Department of General Surgery, Linyi Central Hospital, Linyi, People's Republic of China |
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Abstract: | Ovarian cancer characterizes as the fourth leading consequence of death associated with cancer for women. Accumulating evidence underscores the vital roles of microRNAs (miRNAs) in preventing ovarian cancer development. Besides, induction of the phosphatidylinositol-3 kinase/serine/threonine kinase (PI3K/Akt) pathway associated with the ovarian cancer cell migration and invasion. The study aims to examine the effects of miR-15b on the proliferation, apoptosis, and senescence of human ovarian cancer cells by binding to lysophosphatidic acid receptor 3 (LPAR3) with the involvement of the PI3K/Akt pathway. The positive expression of LPAR3 protein was detected by immunohistochemistry. Then the interaction between miR-15b and LPAR3 was examined. The possible role of miR-15b in ovarian cancer was explored using gain- and loss-of-function experiments. Subsequently, the functions of miR-15b on PI3K/Akt pathway, proliferation, migration, invasion, senescence and apoptosis of ovarian cancer cells were assessed. Furthermore, in vivo tumorigenicity assay in nude mice was performed. LPAR3 was overexpressed, whereas miR-15b was poorly expressed in ovarian cancer tissues. LPAR3 is a direct target of miR-15b. Restored miR-15b promoted Bax expression, apoptosis, and senescence, inhibited expression of LPAR3 and Bcl-2, the extent of PI3K and Akt phosphorylation, as well as ovarian cancer cell proliferation, migration, and invasion. Further, tumor growth was observed to be prevented by miR-15b overexpression. Collectively, our study demonstrates that miR-15b represses the proliferation and drives the senescence and apoptosis of ovarian cancer cells through the suppression of LPAR3 and the PI3K/Akt pathway, highlighting an antitumorigenic role of miR-15b. |
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Keywords: | apoptosis LPAR3 microRNA-15b ovarian cancer PI3K/Akt pathway proliferation senescence |
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