Institution: | 1. Department of Pharmacy, Università “G d'Annunzio” di Chieti-Pescara, Chieti, Italy;2. Department of Pharmacy, Università “G d'Annunzio” di Chieti-Pescara, Chieti, Italy
Molecular Discovery Limited, Middlesex, London, United Kingdom
ISTM – CNR, Perugia, Italy |
Abstract: | Unveiling the events leading to the formation of prion particles is a nowadays challenge in the field of neurochemistry. Pathogenic mutants of prion protein (PrP) are characterized by both an intrinsic tendency to aggregation and scrapie conversion propensity. However, the question about a possible correlation between these two events lasts still unanswered. Here, a multilayered computational workflow was employed to investigate structure, stability, and molecular interaction properties of a dimer of PrPC-E200K, a well-known mutant of the PrP that represents a reduced model of early aggregates of this protein. Based on the combination of molecular dynamics and quantum mechanical approaches, this study provided for an in depth insight of PrPC-E200K dimer in terms of residue-residue interactions. Assembly hypotheses for the early aggregation of PrPC-E200K are paved and compared with PrPSc models reported in the literature to find a structural link between early and late (scrapie) aggregates of this protein. |