Eremomycin的发酵条件优化及发酵产物的分离纯化

研究确定Nocardia orientalis NRRL18098发酵生产eremomycin的最佳工艺条件,以及对发酵产物进行分离纯化并得到eremomycin的纯品。通过正交设计方法优化发酵培养基的组成。采用树脂吸附、中压液相色谱技术相结合的方法对发酵产物进行分离纯化。在优化条件下,eremomycin的摇瓶发酵单位达115 mg/L,提高了63.5%,并采用树脂吸附和中压液相色谱相结合的方法能有效地将eremomycin从发酵液中分离出来,制备获得eremomycin精制品。

Fermentation Conditions Optimization, Isolation and Purification of Eremomycin

The studies are to optimize the fermentation technological condition of Nocardia orientalis NRRL 18098 and to isolate,purify fermentation product and obtain pure eremomycin.Orthogonal design was used to optimize the fermentation condition,resin adsorption combined with medium-pressure liquid chromatography were adopted for isolation and purification of fermentation products.Under the optimized conditions,the fermentation unit of eremomycin in shaking flask reached 115 mg/L increased by 63.5%.Moreover,resin ...