首页 | 本学科首页   官方微博 | 高级检索  
     


High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula
Authors:Ota Shigenori  Miyazaki Shota  Matsuoka Hideo  Morisato Kei  Shintani Yukihiro  Nakanishi Kazuki
Affiliation:GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama, 358-0032, Japan. sig-ota@gls.co.jp
Abstract:Based on the monolithic silica gel materials with hierarchical pore structure and on the SPE devices (MonoTip) developed thereof, a trypsin-immobilized monolithic silica in a pipette tip (MonoTip Trypsin) suitable for digesting proteins has been newly developed. The surface of monolithic silica fixed into the tip was chemically modified with trypsin via an aminopropyl group. Trypsin-immobilized monolith successfully performed a rapid digestion of reduced and alkylated proteins with only a few times pipetting operation for the pre-treatment procedure of chromatographic analysis. The novel solid-phase digestion tool using monolithic silica allows a high-throughput trypsin proteolysis of bio-substances in proteomics.
Keywords:Trypsin-immobilized monolithic silica   Digestion tool with pipette-tip shape   High-throughput digestion   Tryptic mapping   Proteins
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号