Routine identification of Campylobacter jejuni and Campylobacter coli from human stool samples |
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| Authors: | Bernhard Steinbruecknera Georg Haertera Klaus Pelza Manfred Kista |
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| Institution: | 1. College of Longrun Pu-erh Tea, Yunnan Agricultural University, Kunming, 650201, Yunnan, China;2. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, Anhui, China;1. Department of Biophysics, Panjab University, Chandigarh, India;2. Department of Biochemistry, Panjab University, Chandigarh, India;1. Laboratorio di Ricerche Applicate, Pompeii Archaeological Park, Italy;2. Consiglio Nazionale delle Ricerche, Institute of Heritage Science (ISPC), Naples, Italy;3. Laboratory of Anthropology, Molecular Anthropology/Paleogenetics Unit, Department of Biology, University of Florence, Italy;4. Head of State Museums, Ministry of Culture, Italy;1. Department of Chemistry, Indian Institute of Science Education and Research (IISER), Pune 411008, India;2. Department of Physics and Centre for Energy Sciences, Indian Institute of Science Education and Research (IISER), Pune 411008, India;1. Medical Oncology Department, Institut Jules Bordet and l’Université Libre de Bruxelles (U.L.B.), Rue Meylemeersch 90, 1070 Brussels, Belgium;2. Bibliothèque des Sciences de la Santé, Université Libre de Bruxelles (U.L.B.), Route de lennik 808, 1070 Brussels, Belgium;3. Radiation Oncology Department, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi 10, 16132 Genova, Italy;4. Department of Infectious Diseases, CHU Saint-Pierre, Rue haute 322, 1000 Brussels, Belgium;5. School of Public Health, Université Libre de Bruxelles (U.L.B.), Route de lennik 808, 1070 Brussels, Belgium;6. Radiotherapy Unit, ARNAS Civico Hospital – Piazza Nicola Leotta 4, 90100 Palermo, Italy;7. Vrije Universiteit Brussel (V.U.B.), Universitair Ziekenhuis Brussel (UZ Brussel), Department of Medical Oncology, Laarbeeklaan 101, 1090 Brussels, Belgium;8. Advanced Radiation Oncology Department, IRCCS Sacro Cuore Don Calabria Hospital, Via Don A.Sempreboni, 37124 Negrar di Valpolicella, Italy |
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| Abstract: | Correct identification of Campylobacter jejuni and Campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. The widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. This investigation was performed to assess the performance of common biochemical identification in comparison with species-specific PCR and gas liquid chromatography of whole cell fatty acid extracts (GLC). A total of 150 consecutive isolates from human stool samples were investigated (134 C. jejuni ssp. jejuni, 14 C. coli, two Helicobacter pullorum). From these 144, 145 and 149 isolates were correctly identified by biochemistry, GLC and PCR, respectively. Biochemical identification of all C. jejuni isolates was confirmed by PCR. GLC detected both H. pullorum strains but misidentified two C. coli strains as C. jejuni and one C. jejuni strain as C. coli. No single method can be defined as 'gold standard' for identification of C. jejuni and C. coli but a combination of techniques is needed. Therefore a stepwise identification scheme starting with biochemical reactions is suggested. All results other than C. jejuni should be confirmed by further methods. For indoxyl acetate-positive isolates species-specific PCR is recommended while GLC seems to be advantageous in indoxyl acetate-negative isolates. |
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| Keywords: | Campylobacter jejuni Campylobacter coli Gas chromatography Biochemical identification Polymerase chain reaction |
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