Selective 1H-13C NMR spectroscopy of methyl groups in residually protonated samples of large proteins |
| |
Authors: | Chenyun Guo Vitali Tugarinov |
| |
Affiliation: | (1) Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA; |
| |
Abstract: | Methyl 13CHD2 isotopomers of all methyl-containing amino-acids can be observed in residually protonated samples of large proteins obtained from [U-13C,1H]-glucose/D2O-based bacterial media, with sensitivity sufficient for a number of NMR applications. Selective detection of some subsets of methyl groups (Alaβ, Thrγ2) is possible using simple ‘out-and-back’ NMR methodology. Such selective methyl-detected ‘out-and-back’ NMR experiments allow complete assignments of threonine γ2 methyls in residually protonated, [U-13C,1H]-glucose/D2O-derived samples of an 82-kDa enzyme Malate Synthase G. [U-13C,1H]-glucose/D2O-derived protein samples are relatively inexpensive and are usually available at very early stages of any NMR study of high-molecular-weight systems. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|