严重烧伤早期红细胞膜胆固醇含量及Na+-K+-ATP酶活性的变化

目的 :研究严重烧伤患者早期红细胞滤过指数 (EFI)与红细胞膜胆固醇含量、Na K ATPase活性的变化。探讨其在严重烧伤早期中的相互关系及意义。方法 :采用核孔滤膜法测定红细胞滤过指数 (EFI) ,用化学修饰电极法测定红细胞膜胆固醇含量 ,应用定磷法测定红细胞膜Na K ATPase活性。结果 :4 7例严重烧伤早期患者EFI较 6 0例正常对照组下降 (P <0 .0 1) ,红细胞膜胆固醇含量、Na K ATPase活性均高于正常对照组 (P <0 .0 1) ,且红细胞膜胆固醇含量、Na K ATPase活性与EFI呈密切负相关 (rcho =- 0 .871,rATPase =- 0 .80 1,P <0 .0 1)。结论 :严重烧伤早期EFI下降 ,变形性明显减低是导致血液粘度和微循环改变的原因之一 ,红细胞膜胆固醇含量和Na K ATPase活性的变化则是引起EFI下降、变形性减低的重要因素

The change of cholesterol level and Na+ -K+ -ATPase activity of erythrocyte membrane in early stage of severe burn patients

Aim: To study the relationship between erythrocyte filtration index (EFI) and cholesterol and Na K ATPase activity of erythrocyte membrane in early stage of severe burns. Methods: The EFI was measured by means of percolation, the erythrocyte membrane cholesterol level was measured by means of chemically modified doctored, and the membrane Na K ATPase activity by means of detection of Pi. Results: In early stage of severe burns, the cholesterol level and Na K ATPase activity of erythrocyte membrane were always higher than those of control group ( P < 0.01 ), but the EFI was lower than that of control group ( P < 0.01 ). The cholesterol level and Na K ATPase activity of the erythrocyte membrane were negative correlation with EFI (r cho=-0.871,r ATPase=-0.801, P <0.01) nospectively. Conclusion: The cholesterol level and Na K ATPase activity of erythrocyte membrane play a key role in the change of EFI.