两个品系豚鼠对化学介质诱导产生气道反应的差异性研究

目的　研究新育成的Zmu 1∶DHP豚鼠气道对外界化学介质诱导产生的反应性。为研究哮喘选择和提供具有较高敏感性的速发型过敏性动物模型。方法　采用雾化气体吸入法 ,按递增浓度 ,让动物吸入组胺及乙酰胆碱气体 ,记录豚鼠到达哮喘发作时的介质浓度和呼吸频率及幅度 ,评定对化学介质的敏感程度 ,同时用DHP品系豚鼠进行对照。结果　当 0 2 %组胺浓度雾化吸入时 ,Zmu 1∶DHP豚鼠哮喘发作的呼吸频率及每分钟通气量 ,显著大于DHP豚鼠 (P <0 0 5 ) ;当 0 4 %浓度时 ,前者的潮气量及每分钟通气量 ,比后者有增高的趋势 (P <0 0 5 ) ;当0 6 %浓度时 ,前者的潮气量及每分钟通气量 ,显著小于后者 (P <0 0 5 )。二个品系豚鼠吸入乙酰胆碱雾化气体后 ,无明显差异 (P >0 0 5 )。结论　在低浓度组胺雾化吸入时 ,Zmu 1∶DHP品系豚鼠产生哮喘的敏感性显著高于DHP豚鼠 ;高浓度时 ,气道可能因失敏而降低反应性

Study on the Difference of the Chemical Mediator-Induced Airway Reactivity between Two Strains of Guinea Pig

Objective To study the sensibility of airway of new strain Zmu 1:DHP guinea pig inhaling the chemical mediator from the respiratory tract. Method With increasing concentration, histamine (His) and acetylcholine (Ach) were breathed in respectively by the animals, and the time and the mediator concentration of the asthma onset were recorded in comparison with DHP guinea pig. Results When inhaling the gas of 0 02% His, the respiratory rate and the volume of flow of Zmu 1:DHP guinea pig were higher significantly than DHP guinea pig ( P <0 05). When 0 04% His, both of the strains were not divergent remarkably in asthma ( P >0 05). When 0 06% His, these of Zmu 1:DHP guinea pig were lower notably than DHP guinea pig ( P <0 05). Conclusion The sensibility of Zmu 1:DHP guinea pig is higher significantly to the reaction of asthma than DHP guinea pig when the lower His gas is inhaled.