Transfer and expression of the tetracyclin resistance transposon Tn925 in Acetobacterium woodii |
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Authors: | Michael Strä tz,Gerhard Gottschalk,Peter Dü rre |
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Affiliation: | Institut für Mikrobiologie, Universit?t G?ttingen, F.R.G. |
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Abstract: | The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10(-6) per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of HindIII-digested chromosomal DNA demonstrated the insertion of Tn925 at a variety of locations, whereas wild type DNA showed no hybridization at all. The transconjugants were used as donor in mating experiments with tetracycline-sensitive Bacillus subtilis. Transfer of tetracycline resistance was observed at frequencies of 10(-8) per recipient. |
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Keywords: | Acetobacterium woodii Tetracycline resistance transposon Transfer Expression |
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