Evaluation of swollenin from <Emphasis Type="Italic">Trichoderma pseudokoningii</Emphasis> as a potential synergistic factor in the enzymatic hydrolysis of cellulose with low cellulase loadings |
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Authors: | Qingxin Zhou Xinxing Lv Xi Zhang Xiangfeng Meng Guanjun Chen Weifeng Liu |
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Institution: | (1) The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, 250100 Jinan, Shandong, People’s Republic of China; |
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Abstract: | Swollenin is a novel plant expansin-like protein that has been proposed to have a cellulose disruption activity. In this study,
the recombinant swollenin (SWO2) from Trichoderma pseudokoningii S38 was successfully produced and purified in Aspergillus niger with a final yield of up to 10 mg of purified protein from 1 l of fermentation supernatant. The recombinant protein was found
to exhibit very low level of endoglucanase activity and caused a slight increase in the crystallinity when treating cellulose.
Simultaneous incubation of SWO2 with low-dose cellulases resulted in a significant synergistic activity in cellulose hydrolysis.
Specifically, an even greater increase in the synergistic activity was obtained when cellulose was pretreated with swollenin
followed by cellulase hydrolysis. Our results, therefore, provide a novel approach for the potential application of swollenin
in the efficient saccharification of cellulosic materials. |
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