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Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures |
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| Authors: | Tae-Ho Kwon Young-Sook Kim Jae-Hwa Lee Moon-Sik Yang |
| Institution: | (1) Basic Science Research Institute, Chonbuk National University, Jeonju, 561-756, Republic of Korea;(2) Department of Bioscience and Biotechnology, Silla University, Kwaebop-dong 1-1, Pusan, 617-736, Republic of Korea;(3) Basic Science Research Institute, Division of Biological Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea |
| Abstract: | A complementary DNA encoding human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned and introduced into tomato (Lycopersicon esculentum cv. Seokwang) using Agrobacterium-mediated transformation. Genomic PCR and Northern blot analysis demonstrated the integration of the construction into the plant nuclear genome and expression of the hGM-CSF in transgenic tomato. The cell suspension culture was established from leaf-derived calli of the transgenic tomato plants transformed with the hGM-CSF gene. Recombinant hGM-CSF was synthesized by the transgenic cell culture and secreted into the growth medium at 45  g l–1 after 10 d' cultivation. |
| Keywords: | granulocyte-macrophage colony stimulating factor plant suspension culture recombinant protein tomato |
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