Effect of pepstatin A on the virulence factors of Candida albicans strains isolated from vaginal environment of patients in three different clinical conditions |
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Authors: | M. E. L. Consolaro A. Gasparetto T. I. E. Svidzinski R. M. Peralta |
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Affiliation: | (1) Division of Medical Mycology, Teaching and Research in Clinical Analysis Laboratory, State University of Maringá, Maringá, Paraná, Brazil;(2) Department of Odontology, State University of Maringá, Maringá, Paraná, Brazil;(3) Department of Biochemistry , State University of Maringá, Maringá, Paraná, 5790, CEP: 87020-900, Brazil |
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Abstract: | The aspartate proteinase inhibitor pepstatin A was used to study a possible correlation among proteinase activity and other virulence factors of Candida albicans strains isolated from the vaginal environment of patients in three different clinical conditions: asympthomatic, vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC). The addition of 1.0 μM pepstatin A did not have any significant effect on hyphae formation, biofilm production and in the cell surface hydrofobicity of isolates in the three different clinical conditions. However, pepstatin A reduced the adherence of C. albicans to vaginal mucosa epithelial cells (53.1, 48.7 and 59.9%, respectively to isolates from asymptomatic, VVC and RVVC patients). This result suggests that the secreted aspartate proteinases (Saps) of this fungal pathogen may have auxiliary roles in cellular adhesion. |
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Keywords: | Adherence aspartate proteinase Candida albicans virulence factors pepstatin A |
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