Characterization of gastric mucosal membranes. VI. The presence of channel-forming substances |
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| Authors: | G Sachs JG Spenney G Saccomani MC Goodall |
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| Institution: | Division of Gastroenterology and Department of Physiology and Biophysics University of Alabama in Birmingham, Birmingham, Ala. 35294 U.S.A. |
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| Abstract: | Highly purified gastric membranes were extracted by ionic or non-ionic detergents. Further fractionation was carried out on acrylamide gel electrophoresis. Some defined bands, incorporated into artificial bilayer membranes, produced discrete conductance changes characteristics of channel activity. A low molecular weight peak, purified through two cycles of electrophoresis was characterized as an anion-selective, positively voltage dependent channel, and a model to account for the action of the transport ATPase incoporating this channel concept is suggested. |
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| Keywords: | To whom correspondence should be addressed |
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