Inactivation of nitrate reductase by NADH in Nitrobacter agilis |
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Authors: | J Herrera DJD Nicholas |
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Institution: | Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, S.A. 5064 Australia |
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Abstract: | Nitrate reductase from Nitrobacter agilis was inactivated by NADH (but not by NADPH) in the absence of nitrate.The inactivation of the enzyme by over-reduction with NADH was overcome by oxidizing the reduced enzyme with nitrate, ferricyanide, NAD+ or NADP+. |
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