Calcium tips the balance: a microtubule plus end to lattice binding switch operates in the carboxyl terminus of BPAG1n4 |
| |
Authors: | Mridu Kapur Wei Wang Michael T Maloney Ivan Millan Victor F Lundin Thuy‐An Tran Yanmin Yang |
| |
Institution: | Department of Neurology and Neurological Sciences, Stanford University School of Medicine, MSLS Building, Room P259, 1201 Welch Road, , Stanford, California 94305, USA |
| |
Abstract: | Microtubules (MTs) are integral to numerous cellular functions, such as cell adhesion, differentiation and intracellular transport. Their dynamics are largely controlled by diverse MT‐interacting proteins, but the signalling mechanisms that regulate these interactions remain elusive. In this report, we identify a rapid, calcium‐regulated switch between MT plus end interaction and lattice binding within the carboxyl terminus of BPAG1n4. This switch is EF‐hand dependent, and mutations of the EF‐hands abolish this dynamic behaviour. Our study thus uncovers a new, calcium‐dependent regulatory mechanism for a spectraplakin, BPAG1n4, at the MT plus end. |
| |
Keywords: | spectraplakin microtubule plus end calcium EF‐hand |
|
|