Investigation of the significance of Oil Red O‐positive macrophage excess in bronchoalveolar lavage fluid during HIV infection |
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Authors: | L. Lacoste‐Collin G. Martin‐Blondel C. Basset‐Léobon V. Lauwers‐Cancès D. d’Aure J. Aziza A. Berry B Marchou M. B. Delisle M. Courtade‐Saïdi |
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Affiliation: | 1. Department of Pathology and Histology‐Cytology, Toulouse Rangueil Hospital, Toulouse Cedex, France;2. Department of Infectious and Tropical Diseases, Toulouse Purpan Hospital, Toulouse Cedex, France;3. Department of Epidemiology, Toulouse Hospital, Toulouse, France;4. Department of Parasitology, Toulouse Rangueil Hospital, Toulouse Cedex, France |
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Abstract: | L. Lacoste‐Collin, G. Martin‐Blondel, C. Basset‐Léobon, V. Lauwers‐Cancès, D. d’Aure, J. Aziza, A. Berry, B Marchou, M.B. Delisle and M. Courtade‐Saïdi Investigation of the significance of Oil Red O‐positive macrophage excess in bronchoalveolar lavage fluid during HIV infection Objective: To assess the significance of increased levels of Oil Red O‐positive macrophages (ORO‐PM) in bronchoalveolar lavage fluids (BALFs) from HIV‐positive patients. Methods: Cytological data for seventy BALF samples from 66 consecutive HIV‐infected patients were analysed according to antiretroviral therapy regimen, presence of Pneumocystis jiroveci infection, blood CD4+T cell count, HIV‐1 viral load and plasma lipid levels. Non‐parametric tests were used to compare the values between groups. Results: The percentages of ORO‐PM were high in this group: 40% [6–80] (median [interquartile range]). They were positively correlated with the BALF total cell count, 21% [5–48.5] for <300 cells/mm3 and 60% [26.5–80] for >300 cells/mm3 (P < 0.01) but inversely correlated with the percentage of BALF lymphocytes, 50% [20–80] for <15% lymphocytes and 11.5% [2–47] for ≥15% lymphocytes (P < 0.01). Antiretroviral therapy with or without protease inhibitors, plasma lipid levels, HIV‐1 viral load, blood CD4+T cell count or presence of a Pneumocystis jiroveci infection were not correlated with the ORO‐PM status. Conclusion: Significantly increased numbers of ORO‐PM were correlated with high total cell counts and low lymphocyte counts in BALF, irrespective of disease activity or treatment. Extended work on a larger series of patients needs to be conducted. |
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Keywords: | bronchoalveolar lavage fluid alveolar macrophages Oil Red O cytology HIV |
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