Structural studies of a mitochondria-free plasma membrane fraction from rat liver |
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| Authors: | Nicholas Wong James E. Zull |
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| Affiliation: | Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A. |
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| Abstract: | Liver plasma membranes virtually free of contaminating mitochondria have been prepared. Sodium dodecylsulfate-polyacrylamide gel electrophoresis reveals a membrane protein resistant to papain digestion in the intact membranes but readily hydrolyzed in membranes disrupted by detergent or sonication.Electron microscopy of mechanically deformed membranes reveals fibrils within the membrane which appear to be protein in nature but which also persist in papain digested membranes. |
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| Keywords: | To whom correspondence should be sent. |
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