The effect of ionic strength on a Mg2+-ATPase and its relevance to the determination of (Na++K+)-ATPase |
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| Authors: | Kenneth T Izutsu Ivens A Siegel Douglas L Brisson |
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| Institution: | Departments of Oral Biology and Pharmacology, and Center for Research in Oral Biology, University of Washington, Schools of Dentistry and Medicine, Seattle, Wash. 98195 U.S.A. |
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| Abstract: | A ouabain-insensitive Mg2+-ATPase present in a microsomal fraction prepared from the dog submandibular gland was studied. This Mg2+-ATPase was inhibited by increasing concentrations of NaCl, KCl, RbCl and CsCl. The addition of an osmotically equal amount of sucrose was without effect. This inhibition was obtained over a pH range of from 6.3 to 8.8. The Mg2+-ATPase present in microsomes treated with NaI showed a similar inhibition. These results indicate that it is advisable to keep the ionic strength constant in solutions used to obtain (Na++K+)-ATPase activities. |
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| Keywords: | 12-nitroxide stearate 2-(10-carboxydecyl)-2-hexyl-4 4-dimethyl-3-oxazolidinyloxyl |
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