Assessment of Gene Flow from Genetically Modified Anthracnose-Resistant Chili Pepper (Capsicum annuum L.) to a Conventional Crop |
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Authors: | Chang-Gi Kim Dae In Kim Hyo-Jeong Kim Ji Eun Park Bumkyu Lee Kee Woong Park Soon-Chun Jeong Kyung Hwa Choi Joo Hee An Kang-Hyun Cho Young Soon Kim Hwan Mook Kim |
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Institution: | (1) Bio-Evaluation Center, KRIBB, 685-1 Yangcheong-ri, Ochang-eup, Cheongwon-gun, Chungcheongbuk-do, 363-883, Republic of Korea;(2) Biosafety Division, National Academy of Agricultural Science, RDA, Suwon, 441-707, Republic of Korea;(3) Department of Biological Sciences, Inha University, Incheon, 402-751, Republic of Korea;(4) Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, 500-712, Republic of Korea |
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Abstract: | We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line “WT512” and two commercial hybrid cultivars, “Manidda” and “Cheongpung Myeongwol
(CM).” After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened
by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 “WT512” seeds and
6,854 “Manidda” seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456
“WT512” seeds and 50 hybrids from 3,457 “CM” seeds were found. The highest frequency of gene flow, 6.19%, was observed in
that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM
to conventionally bred chili peppers. |
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Keywords: | Capsicum annuum Chili pepper Gene flow Genetically modified (GM) crop |
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