The binding of actinomycin D and adriamycin to supercoiled DNA, single-stranded DNA and polynucleotides |
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Authors: | H. Simpkins L.F. Pearlman |
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Affiliation: | Departments of Pathology and Biological Chemistry, University of California, Irvine, Irvine, CA 92717 U.S.A. |
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Abstract: | The effect of actinomycin D and adriamycin on synthetic polynucleotides, single-stranded viral DNA and supercoiled DNA has been studied employing the fluorescent probe, terbium. Marked displacement of the probe was observed when any deoxyribose-containing polynucleotide was pretreated with either drug. With supercoiled DNA, an unwinding of the supercoil was observed at very low drug concentrations (at approx. 1:500 molar ratio of drug:DNA) prior to the displacement of the terbium. This unwinding was visualized by agarose gel electrophoresis at molar ratios of approx. 1:200. The effect was more apparent and occurred at lower drug:DNA ratios with actinomycin D than with adriamycin. Unlike cis-dichlorodiammine platinum(II), actinomycin D did not protect pBR322 DNA from cleavage at its BamHI site. The hydrolysis of Φχ174 DNA by a series of G-C-specific restriction nucleases (including HhaI, HpaII and HaeIII) was also not affected by prior treatment of the DNA with actinomycin D. |
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Keywords: | Actinomycin D Adriamycin DNA structure Fluorescence Antibiotic-DNA interaction |
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