Pyrrolidine dithiocarbamate induces cyclooxygenase-2 expression in NIH 3T3 fibroblast cells |
| |
Authors: | Lee Jeong Eun Kim Kyung Min Cho Jae-We Suh Seong-Il Suh Min-Ho Kwon Taeg Kyu Park Jong-Wook Bae Jae Hoon Song Dae Kyu Cho Chi-Heum Bae Insoo Baek Won-Ki |
| |
Institution: | Department of Microbiology, Keimyung University School of Medicine, 194 Dongsan-Dong, Taegu 700-712, Republic of Korea. |
| |
Abstract: | Pyrrolidine dithiocarbamate (PDTC) is a metal chelating compound that can exert either pro-oxidant or antioxidant effects in different situations. Several studies demonstrate that it can inhibit cyclooxygenase-2 (COX-2) expression, which may be due to its antioxidant activity. Here, we found that PDTC rather increased COX-2 expression in NIH 3T3. The increase of COX-2 expression was inhibited by adding bathocuproline disulfonic acid, a non-permeable specific copper chelator, in the incubation medium. This result suggests that PDTC exerts its effect by transporting redox-active copper ions into the cells. In support of this observation, PDTC did not induce COX-2 expression in a serum-free environment. When PDTC was added with copper in the serum-free medium, it acted as the inducer of COX-2 expression. In addition, pretreatment of N-acetyl-L-cystein or dithiothreitol, other antioxidants, inhibited the PDTC-induced COX-2 expression. Our data indicate that PDTC induces COX-2 expression in NIH 3T3 cells, which may be due to its activities as a copper chelator and a pro-oxidant. |
| |
Keywords: | Pyrrolidine dithiocarbamate Cyclooxygenase-2 Copper Pro-oxidant Fibroblast |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|