Development of a gradient reversed-phase HPLC method for the determination of sodium ferulate in beagle dog plasma |
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Authors: | Li Feng-Qian Xu Shu Su Hua Deng Jia-Xin Liu Ji-Yong Hu Jin-Hong |
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Institution: | Department of Pharmaceutical Sciences, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. |
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Abstract: | A gradient reversed-phase HPLC assay has been developed to determine sodium ferulate (SF) in beagle dog plasma with tinidazole as an internal standard. Chromatographic separation was made on a C(18) column using 0.5% acetic acid and acetonitrile (80:20, v/v) as mobile phase. UV detection was performed at 320 nm. The calibration curve for SF was linear in the range of 0.05-10 microg/ml, and the achieved limit of quantification (LOQ) was 51.4 ng/ml. The results of linearity, within- and between-day precision, and accuracy demonstrate that this method is reliable, sensitive and sufficient for in vivo beagle dog pharmacokinetic (PK) studies of SF. |
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