Radioimmunoassay of nicotine. |
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| Authors: | H Matsuyama T Tamada T Ishiguro H Muranaka |
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| Affiliation: | Third Division, Department of Medicine, Kobe University School of Medicine, Ikuta-ku, Kobe, Japan;Kyoto Hospital of The Japan Tobacco & Salt Public Corporation, Higashiyama-ku, Kyoto, Japan |
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| Abstract: | A sensitive and specific radioimmunoassay of nicotine was developed using antisera raised against 6-(p-aminobenzamido) nicotine coupled to bovine serum albumin. Inhibition studies with various nicotine analogues revealed that the antisera are highly specific for both the N-methylpyrrolidine ring and the pyridine ring of nicotine, and especially for the structural changes of the former. The use of these antisera in an assay of nicotine in biological fluids is desirable, since the pyrrolidine ring of nicotine is first metabolized in vivo and antibodies must, therefore, discriminate nicotine from other nicotine metabolites. |
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