A phycourobilin-containing phycoerythrin from the cyanobacterium Oscillatoria sp. |
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Authors: | Igor N Stadnichuk Natalya I Romanova Irina O Selyakh |
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Institution: | (1) School of Biology, Lomonosov State University, 119899 Moscow, USSR |
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Abstract: | A filamentous cyanobacterium Oscillatoria sp. was isolated from a thermal spring of the Kamchatka peninsula. It contained a phycoerythrin unusual for cyanobacteria in that it had a phycourobilin prosthetic group. The absorption spectrum of the native purified phycoerythrin displayed maxima at 498 and 567 nm. The phycoerythrin comprised - and -subunits of molecular weights 18,700 and 19,800, respectively, in 1:1 stoichiometry. Polyacrylamide gel isoelectric focusing revealed one protein band at pI 4.6. The - and -subunits differed in their chromophore composition and content: -subunit carried two phycoerythrobilins while the -subunit had three phycoerythrobilins and one phycourobilin. The chromophore composition of all known phycoerythrins of cyanobacteria and red algae were compared, and on the basis of this comparative study designations C1- to C5-phycoerythrin were proposed for cyanobacterial red pigments. |
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Keywords: | Oscillatoria sp Phycoerythrin(s) Phycobiliprotein(s) Phycoerythrobilin Phycourobilin |
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