Monoclonal antibodies inhibiting RNA polymerase from Escherichia coli |
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| Authors: | V G Nikiforov L Z Yakubov G T Bogachova A N Lebedev O V Rokhlin |
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| Affiliation: | 1. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow 123182 USSR;2. National Cardiological Research Center, USSR Academy of Medical Science, Moscow, USSR |
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| Abstract: | Monoclonal hybridoma antibodies directed against RNA polymerase from E. coli have been obtained. Only a few have been found to inhibit the enzyme activity. Antibodies produced by two clones, PYN-1 and PYN-2, inhibit RNA polymerase at the stage of RNA chain elongation. The PYN-1 antibodies react with the beta'-subunit of the enzyme. The PYN-2 antibodies react with the beta-subunit and with its 130 kDa amber fragment. |
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| Keywords: | RNA polymerase RNA chain elongation Monoclonal antibody Immunoblotting |
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