A possible rate limiting factor in urea synthesis by isolated hepatocytes: the transport of ornithine into hepatocytes and mitochondria |
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| Authors: | K Metoki F A Hommes |
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| Institution: | 1. Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT, USA;2. Division of Hematology, Department of Internal Medicine, School of Medicine, University of Utah, Salt Lake City, UT, USA;3. Biology Department, Al Azhar University-Gaza, Palestine;4. University of Utah School of Medicine, Salt Lake City, UT, USA;5. Department of Laboratory Medical Sciences, Islamic University-Gaza, Palestine;6. Al Nasser Pediatric Hospital, Palestinian Ministry of Health, Palestine;7. Maternity Hospital, Al Shifa Medical Compound, Palestinian Ministry of Health, Palestine;8. Department of Hematology, Muhimbili University of Health and Allied Sciences, Dar-es-Salaam, Tanzania;1. Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Gründenstrasse 40, CH-4132 Muttenz, Switzerland;2. Institute of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland;3. AgroBioInstitute, Agriculture Academy, Dragan Tzankov 8, Sofia 1164, Bulgaria |
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| Abstract: | The uptake of ornithine by isolated hepatocytes and by the particulate fraction of these cells was measured under various conditions of urea synthesis. Under conditions of maximum urea synthesis, i.e. in the presence of glucose, ornithine, ammonium chloride and oleate, the cytosolic concentration and the mitochondrial concentration of ornithine was extremely low, while citrulline accumulated in the cytosol. The data indicate that the rate of citrulline synthesis is limited by the availability of mitochondrial ornithine. |
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