Heterochromatin characterization of sex chromosomes in Triturus marmoratus (Urodela, Salamandridae). |
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| Authors: | P Herrero J de la Torre J Gosálvez A T Sumner |
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| Institution: | Unidad de Fisiología Animal, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain. |
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| Abstract: | The sex chromosomes of the Iberian marbled newt, Triturus marmoratus, were studied using various banding techniques, including restriction enzyme/nick translation (RE/NT) procedures. Four types of heterochromatin on the sex chromosomes could be distinguished: (1) distamycin A/DAPI and chromomycin A3/distamycin A positive, EcoRI/NT negative, and HaeIII/NT and HinfI/NT positive; (2) distamycin A/DAPI and chromomycin A3/distamycin A positive, but RE/NT negative; (3) AT rich, but RE/NT negative; and (4) distamycin A/DAPI and chromomycin A3/distamycin A positive, EcoRI/NT and HinfI/NT negative, but HaeIII/NT positive. These data suggest a common origin for the terminal heterochromatic domains of both the X and Y chromosomes in this species. |
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