Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins |
| |
Authors: | Horn German P Vongpunsawad Sompong Kornmann Evelyn Fritz Barbara Dittmer Dirk P Cattaneo Roberto Dobbelstein Matthias |
| |
Institution: | Institut für Virologie, Klinikum der Philipps, Universit?t Marburg, Marburg, Germany. |
| |
Abstract: | The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|