中国东乡族9个STR基因座遗传多态性研究

选择9个STR基因座，采用四色荧光标记STR基因扫描技术，对中国甘肃省特有民族——东乡族的群体遗传多态性进行研究。同时检测94个无关个体血液样本，共检出72种等位基因，基因频率的分布在0.0053~0.5825之间；检出182种基因型，基因型频率分布在0.0106~0.2660之间；9个STR位点基因型分布均符合Hardy-Weinberg平衡定律（P＞0.05）。9个STR位点多态信息量（polymorphism information content,PIC）均大于0.6378，杂合度(heterozygosity，H) 均大于0.6500，个体识别力(discrimination power,DP)均大于0.8216，非父排除率(probabilities of paternity exclusion,PPE) 均大于 0.4903。种族比较结果显示，甘肃东乡族与白种人及黑种人在绝大多数位点存在显著差异（P＜0.05），而9个STR位点与汉族群体的遗传差异均不显著（P＞0.05）。研究结果丰富了中华民族基因数据库，在人类群体遗传学及法医学研究领域有重要应用价值。Abstract:Genetic distribution for nine STR loci was determined in a Chinese Dongxing ethnic group based on STR genescan marked by fluorescence.Seventy-Two alleles and 182 genotypes were observed in 94 unrelated Chinese Dongxiang individuals,with the corresponding gene frequency and genotype frequency being 0.0053~0.5825 and 0.0106~0.2660 respectively.The genotypes of nine STR loci were in accordance with the Hardy-Weinberg equilibrium (P＞0.05).The statistical analysis of nine STR loci showed PIC( polymorphism information content,PIC)≥0.6378,H(heterozygosity,H) ≥0.6500,DP (discrimination power,DP) ≥0.8216,PPE(probabilities of paternity exculation,PPE) ≥0.4903.The result indicated that there was a significant difference between Dongxiang ethnic group and the white and the black.There was no significant difference in Han nationality.These result filled the Dongxiang ethnic group-a specific group of Chinese into the genetic database and played an important role in Chinese population genetic study and forensic medicine application.

Genetic Polymorphisms of 9 STR Loci in Dongxiang Ethnic Group of China

Genetic distribution for nine STR loci was determined in a Chinese Dongxing ethnic group based on STR genescan marked by fluorescence. Seventy-two alleles and 182 genotypes were observed in 94 unrelated Chinese Dongxiang individuals,with the corresponding gene frequency and genotype frequency being 0.0053-0.5825 and 0.0106-0.2660 respectively. The genotypes of nine STR loci were in accordance with the Hardy-Weinberg equilibrium (P>0.05). The statistical analysis of nine STR loci showed PIC (polymorphism information content, PIC) = or > 0.6378, H(heterozygosity, H) = or > 0.6500, DP (discrimination power, DP) = or > 0.8216, PPE (probabilities of paternity exculation, PPE) = or > 0.4903. The result indicated that there was a significant difference between Dongxiang ethnic group and the white and the black. There was no significant difference in Han nationality.These result filled the Dongxiang ethnic group-a specific group of Chinese into the genetic database and played an important role in Chinese population genetic study and forensic medicine application.