Efficient rooting for establishment of papaya plantlets by micropropagation |
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Authors: | Yu Tsong-Ann Yeh Shyi-Dong Cheng Ying-Huey Yang Jiu-Sherng |
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Institution: | (1) Department of Botany, National Chung-Hsing University, Taichung, Taiwan, Republic of China;(2) Department of Plant Pathology, National Chung-Hsing University, Taichung, Taiwan, Republic of China |
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Abstract: | A low cost micropropagation protocol to produce high quality root systems which are easy and economical to acclimatize is
essential for large-scale micropropagation of papaya (Carica papaya L.). In this study, individual shoots (>0.5 cm) with 2∼3 leaves from in vitro papaya multiple shoots were cultured on MS agar medium containing 2.5 μM IBA under dark conditions for 1 week for root induction.
They were then transferred to agar or vermiculite media, containing half strength MS medium, under aerated or non-aerated
conditions, for root development. Rooting percentage of shoots cultured for 2 weeks in aerated vermiculite was 94.5%, compared
with 90.0% in non-aerated vermiculite, 71.1% in aerated agar, and 62.2% in non-aerated agar. Shoots with roots were acclimated
in vermiculite under 100% RH for 1 week and then under ambient conditions for 2 weeks in a temperature-controlled growth chamber
(28 °C). The survival rates of the plantlets were 94.5% from aerated vermiculite, 87.8% from non-aerated vermiculite, 42.2%
from aerated agar, and 35.6% from non-aerated agar. Thus, root induction in low-concentration IBA agar medium followed by
root development in vermiculite containing half strength MS medium under aerated conditions results in efficient rooting of
in vitro papaya shoots.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | aeration agar papaya rooting plantlet survival vermiculite |
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