Extraction of proteins and peptides from Coomassie Blue-stained sodium dodecyl sulfate--polyacrylamide gels. |
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| Authors: | D R Gibson R W Gracy |
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| Institution: | 1. Department of Chemistry, North Texas State University Health Sciences Center Fort Worth, Texas 76107 USA;2. Department of Biochemistry, North Texas State University Health Sciences Center Fort Worth, Texas 76107 USA;3. Texas College of Osteopathic Medicine, Fort Worth, Texas 76107 USA |
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| Abstract: | A method is described for extracting proteins and peptides from stained sodecyl sulfate-polyacrylamide gels. Coomassie blue and sodium dodecyl sulfate present in stained gel sections are removed to allow subsequent analysis of the peptides (e.g., amino acid analysis or tryptic digestion and fingerprinting). The method is simple, requires no radioisotopes or special equipment, and can be carried out with a minimum of handling of the sample. The process can be used for samples at the nanomole level with recoveries of 90%. |
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