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Limited Ca and PKA-pathway dependent neurogenic differentiation of human adult mesenchymal stem cells as compared to fetal neuronal stem cells |
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| Authors: | Guilherme Lepski Cinthia Elim Jannes |
| Affiliation: | a Department of Neurosurgery, Eberhard-Karls-Univesity, Hoppe-Seyler-Str 3, 72076 Tübingen, Germany b Laboratory of Experimental Neurosurgery, Eberhard-Karls-Univesity, Hoppe-Seyler-Str 3, 72076 Tübingen, Germany c Department of Neurosurgery, Neurocentre, Albert-Ludwig University, Breisacherstrasse 64, 79106 Freiburg, Germany d Department of Orthopaedic and Trauma Surgery, University Medical Centre, University of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany e Department of Haematology/Oncology, University Medical Centre, Univeristy of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany f Division of Functional Neurosurgery, Medical School, University of São Paulo, R. Ovidio Pires de Campos 785, 01060-970 São Paulo, SP, Brazil g Laboratory of Molecular Biology LIM15, Medical School, University of São Paulo, Av. Dr. Arnaldo Vieira de Carvalho 455, 01246-093 São Paulo, SP, Brazil h Institute of Physiology I, Albert-Ludwig University, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany i Department of Stereotactic and Functional Neurosurgery, Neurocentre, Albert-Ludwig Universitiy, Breisacherstrasse 64, 79106 Freiburg, Germany |
| Abstract: | |
| Keywords: | NSCs, fetal-derived neural stem cells MSCs, mesenchymal stem cells cAMP, cyclic adenosine monophosphate PKA, protein kinase A CREB, cAMP response element binding protein NeuN, neuronal-specific nuclear protein MAP2, microtubule-associated protein 2 NF-M, neurofilament of medium molecular weigh NF-200, neurofilament with 200KD βIII tubulin the same as Tuj1, microtubule protein TTX, tetrodotoxin FACS, fluorescence-activated cell sorting CD, cluster of differentiation PBS, phosphate-buffered saline bFGF, basic fibroblast growth factor EGF, epidermal growth factor DMEM, Dulbecco's modified Eagle medium F12, Ham's culture medium B27, N2, serum supplements PDGF, platelet-derived growth factor GDNF, glial-derived neurotrophic factor NGF, nerve growth factor IBMX, 3-isobutyl-1-methylxanthine BDNF, brain-derived neurotrophic factor TGFβ, transforming growth factor beta FGF8, fibroblast growth factor 8 NT3, neurotrophin 3, LIF, leukaemia inhibitory factor BrdU, bromodeoxyuridine PFA, paraformaldehyde DAPI, 4,6-diamidino-2-phenylindole dihydrochloride PSA-NCAM, polysialylated neuronal cell adhesion molecule GFAP, glial fibrillar acidic protein GABA, gamma aminobutyric acid ACSF, artificial cerebral spinal fluid |
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