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Limited Ca and PKA-pathway dependent neurogenic differentiation of human adult mesenchymal stem cells as compared to fetal neuronal stem cells |
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| Authors: | Guilherme Lepski Cinthia Elim Jannes |
| Institution: | a Department of Neurosurgery, Eberhard-Karls-Univesity, Hoppe-Seyler-Str 3, 72076 Tübingen, Germany b Laboratory of Experimental Neurosurgery, Eberhard-Karls-Univesity, Hoppe-Seyler-Str 3, 72076 Tübingen, Germany c Department of Neurosurgery, Neurocentre, Albert-Ludwig University, Breisacherstrasse 64, 79106 Freiburg, Germany d Department of Orthopaedic and Trauma Surgery, University Medical Centre, University of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany e Department of Haematology/Oncology, University Medical Centre, Univeristy of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany f Division of Functional Neurosurgery, Medical School, University of São Paulo, R. Ovidio Pires de Campos 785, 01060-970 São Paulo, SP, Brazil g Laboratory of Molecular Biology LIM15, Medical School, University of São Paulo, Av. Dr. Arnaldo Vieira de Carvalho 455, 01246-093 São Paulo, SP, Brazil h Institute of Physiology I, Albert-Ludwig University, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany i Department of Stereotactic and Functional Neurosurgery, Neurocentre, Albert-Ludwig Universitiy, Breisacherstrasse 64, 79106 Freiburg, Germany |
| Abstract: | |
| Keywords: | NSCs fetal-derived neural stem cells MSCs mesenchymal stem cells cAMP cyclic adenosine monophosphate PKA protein kinase A CREB cAMP response element binding protein NeuN neuronal-specific nuclear protein MAP2 microtubule-associated protein 2 NF-M neurofilament of medium molecular weigh NF-200 neurofilament with 200KD βIII tubulin the same as Tuj1 microtubule protein TTX tetrodotoxin FACS fluorescence-activated cell sorting CD cluster of differentiation PBS phosphate-buffered saline bFGF basic fibroblast growth factor EGF epidermal growth factor DMEM Dulbecco's modified Eagle medium F12 Ham's culture medium B27 N2 serum supplements PDGF platelet-derived growth factor GDNF glial-derived neurotrophic factor NGF nerve growth factor IBMX 3-isobutyl-1-methylxanthine BDNF brain-derived neurotrophic factor TGFβ transforming growth factor beta FGF8 fibroblast growth factor 8 NT3 neurotrophin 3 LIF leukaemia inhibitory factor BrdU bromodeoxyuridine PFA paraformaldehyde DAPI 4 6-diamidino-2-phenylindole dihydrochloride PSA-NCAM polysialylated neuronal cell adhesion molecule GFAP glial fibrillar acidic protein GABA gamma aminobutyric acid ACSF artificial cerebral spinal fluid |
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