Intracellular appearance of nitrite and nitrate in nitrogen-starved cells of Ankistrodesmus braunii |
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Authors: | Hartmut Spiller Elisabeth Dietsch Erich Kessler |
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Institution: | (1) Institut für Botanik der Universität, Schloßgarten 4, D-8520 Erlangen, Federal Republic of Germany;(2) Present address: Fachbereich Biologie der Universität, D-7750 Konstanz, FRG |
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Abstract: | Summary The occurrence of heterotrophic nitrification in nitrogen-starved cells of Ankistrodesmus braunii was confirmed. The levels of nitrate and nitrite were measured over a period of four weeks. The validity of quantitative determinations in the presence of highly active nitrate and nitrite reductases is discussed. Whereas free hydroxylamine as an intermediate could not be detected, increased hydroxylamine oxidase activity was found in nitrogen-starved cultures. Nitrite reductase and hydroxylamine oxidase can be assigned to particles by sucrose density gradient centrifugation. The possible involvement of microbodies, which were found to be present in Ankistrodesmus, in metabolic processes during nitrogen starvation is discussed.Abbreviations NR
nitrate reductase
- NiR
nitrite reductase
- NNEDA
N-(1-naphthyl)ethylenediaminedihydrochloride
- DCPIP
2,6-dichlorophenolindophenol
- EDTA
ethylenediaminetetraacetic acid
- TCA
trichloroacetic acid
- DAB
3,3 -diaminobenzidine
- AT
3-amino-1H-1,2,4-triazole
- AMP
2-amino-2-methyl-1,3-propanediol |
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